Chemiluminescent determination of inorganic phosphorus in serum using enzymatic reaction.

Colorimetric vs enzymatic determination of serum phosphorus.

Enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine.

The performance of an enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine is described. Phosphate ions react with inosine in the presence of purine nucleoside phosphorylase to form hypoxanthine; this is oxidized by xanthine oxidase to uric acid with production of hydrogen peroxide. The latter is determined with the aid of the chromogen system peroxidas...

the effect of oxytetracycline on serum calcium, phosphorus and magnesium in cattle

نتایج این مطالعه نشان می دهد که مقدار کلسیم (یونیزه و تام)، منیزیم و فسفر به طور معنی داری تغییر پیدا کرد به جزء مقدار کلسیم تام و منیزیم در روش داخل عضلانی که تغییر معنی داری نداشته است. بر اساس نتایج این مطالعه می توان نتیجه گیری کرد که اکسی تتراساکلین بر روی مقادیر سرمی کلسیم و منیزیم تأثیر می گذارد باید مقدار این کاتیون ها در بیماران، در هنگامی که اکسی تتراسایکلین استفاده می شود مورد توجه ق...

Direct determination of inorganic phosphorus in serum with a single reagent.

Estimation of inorganic phosphate in serum based on the formation of "molybdenum blue" may be simplified by eliminating the reduction stage. The yellow complex formed by the reaction of phosphate with molybdate in an acid medium is measured at 390 nm. The yellow complex behaves in accordance with Beer's law over a wide concentration range (to at least 80 mg of P per liter in the initial sample)...

Enzymatic method for determination of inorganic phosphate in serum and urine with a centrifugal analyzer.

We describe an enzymatic method for determining inorganic phosphate in serum and urine, with use of the “CentrifiChem.” The sample is mixed with a combined enzyme-substrate system consisting of the enzymes phosphorylase a, phosphoglucomutase, and glucose-6-phosphate dehydrogenase, the coenzyme NADP, the substrate glycogen, and adenosine 5’-monophosphate as the activator for phosphorylase. The i...